As one of three core laboratories in the RCMI program, the Cell, Molecular and Bioinformatics Core (CMB Core) will continue to provide “state of the art” comprehensive cell and molecular biology as well as in vitro bioassay support to a wide range of biomedical research projects at Xavier University. The core will further expand its capabilities to include Next-Generation sequencing, antibody Arrays and cell based pharmacokinetics assays. With a newly-added bioinformatics facility, the core will also be able to provide comprehensive statistical, genomic and pathway analysis services. The long term goal of the CMB Core is to improve the biomedical research capacity of Xavier University by enhancing the capabilities of biomedical research projects and programs on campus. To achieve this goal, the CMB Core has three specific aims:
- Provide cell, molecular and bioinformatics support services to faculty research projects
- Provide direct support to the RCMI Major Instrument and Drug Discovery and Delivery Cores.
- Provide technical training and education to researchers at Xavier and local institutions.
Cell and Molecular Services
- Toxicity Assay
- Proliferation Assay
- Cell Viability Assay
- Dose Response - IC50
- Optimize assay from literature
- Maintain Cell and plate as requested
- Dose cells
- Transfections/stable cell lines
- RNAi or shRNA
- Cell line verification services
- 3-D cell culture
- Real time rt-PCR
- Commercial plates
- Pathway/ disease specific
- miRNA screen
- Custom real time rt-PCR
- PCR Array
- DNA Methylation Array
- DNA Somatic Mutation Array
- Protein extraction, purification and quantification
- DNA/ RNA extraction, purification and quantification
- primer design
- Recombinant Protein expression and purification
- cell culture
- Gel electrophoresis and Western Blotting iblot transfer; Proteome profiler panels/other antibody panels
- Immunoprecipitation/ affinity interactions
- Kinase Assay - IC50
- Mass Spectrometry based Global Scale Protein Profiling (major instruments core)
Pathway analysis and custom pathway creation (IPA, String Pathway Analysis freeware, Karen Zhang)
- Antibody Arrays
- Applied bioinformatics and biostatistics services
- Pathway and gene ontology/enrichment analysis
- Analysis of next generation sequencing data
- Network-based systems biology modeling for biomarker identification
- Novel data mining/machine learning algorithm development and pipeline creation
RCMI CMB Pamphlet - Full List of Services Offered with general pricing (PDF).
To request services please follow the iLab Solution link below.
If you want instructions on how to set up an iLab account and submit a request click here.
Important Information for Core Users:
We hope the RCMI Cell, Molecular and Bioinformatics Core (CMB Core) is serving your research needs. As you are writing manuscripts, preparing presentations, and drafting grant applications that have resulted from core usage, please keep in mind the importance of citing the RCMI grant and following the NIH Public Access Policy. In order for a publication or presentation to be counted as productivity towards the RCMI Grant, it must be cited with the grant number and a publication must be deposited into PubMed Central.
Citations must credit NIMHD’s support. Please use the statements below when citing research that was completed with the assistance of an RCMI Core facility. If you are writing RCMI instrumentation into a grant, or using preliminary results obtained with the assistance of an RCMI Core facility, please cite RCMI in the grant application.
“Support is provided in part by grant number 2G12MD007595-06 from the National Institute on Minority Health and Health Disparities (NIMHD), National Institutes of Health (NIH), Department of Health and Human Services (DHHS) and its contents are solely the responsibility of the authors and do not necessarily represent the official views of NIMHD or NIH.”
Obtaining a PubMed Central (PMCID) (This is different than a PMID!)
As a result of NIH's Public Access Policy , the final, peer reviewed author manuscripts of journal articles that are supported by NIH funding must be deposited into PMC via the NIH Manuscript Submission System (NIHMSS) , as soon as the articles have been accepted for publication. Specifically, the final manuscript supplied to PMC is the version that the journal has accepted for publication, including any revisions that the author has made during the peer review process.
You may deposit your final, peer reviewed manuscript through the Manuscript Submission System http://www.nihms.nih.gov/ , using your eRA Commons login, NIH login, HHMI login, or My NCIB login. There are FAQs at the bottom of the webpage and submission tutorials are also available. If you do not have access to one of these logins, please contact Erica Severan for assistance with submission.
PubMed Central: http://www.ncbi.nlm.nih.gov/pmc/
NIH Manuscript Submission System: http://www.nihms.nih.gov/
NIH Public Access Policy: http://publicaccess.nih.gov/
Thank you for helping us stay in compliance with the RCMI Grant and the NIH Public Access Policy. Should you have any questions, please contact the Core Manager, Erica Severan or Guangdi Wang.